Сер.: Рыбное хозяйство. 2014. № 2 ТЕХНОЛОГИЯ ПЕРЕРАБОТКИ ГИДРОБИОНТОВ UDC 606:664.952/.957 A. S. Vinnov, N. V. Dolganova APPLICATION OF FOSTER – NUEMANN METHOD FOR ESTIMATION OF EFFICIENCY OF PROTEIN ENZYMATIC HYDROLYSIS OF BLACK SEA ATHERINA Abstract. <...> The estimation of efficiency of technological modes of protein enzymatic hydrolysis of the black sea atherina by Foster – Nuemann method is made. <...> By the linearization of experimental curves the apparent Michaelis’s constant for substrates with different moisture based on the black sea atherina muscle tissue was obtained. <...> From the experimental Michaelis’s constant values comparison, it follows that in a system with high humidity Protosubtilin G3x enzymes affinity to substrate proteins is higher than in natural humidity systems. <...> The obtained value of Michaelis constant for the theoretical process allows to assert that the enzymatic hydrolysis of the considered enzyme-substrate systems with the addition of 50 % of water does not meet any damping. <...> Key words: fish protein hydrolysates, objective estimation of enzymatic hydrolysis damping level, black sea atherina, Foster – Nuemann method, Michaelis – Menten integral equation. <...> Introduction Fish protein hydrolysates production and their application in feed, food and specialty products allows to solve the problem of raw materials complex processing and to improve fish processing enterprises economic efficiency [1]. <...> Enzyme-substrate system on raw fish basis are viscous mixtures, which consists of dissolved, dispersed and emulsified protein and fat components. <...> Proteins hydrolysis in such multiple substrates associated with a complex inhibitory factors, among which the most probable diffusion damping, individual enzymes competitive inhibition by reaction products, specific substrates depletion, etc. <...> This problem decision allows to select the most effective proteolyses process modes [2]. <...> An objective assessment of enzymatic hydrolysis damping can be obtained from the analysis of experimental kinetic dependences presented in linearized Michaelis – Menten integral equation by Foster – Nuemann method [3]. <...> All considered experimental enzyme-substrate modeling systems must have equal protein / enzyme proportion and different mass fraction of added water. <...> Integral form of the Michaelis – Menten equation has the form: ∑ [ ] = − P V't K' lnm ∑ ∑ , [ ] [ ] 0 S0 <...>