BestuzhevaVeterinдrplatz, A-1210, Vienna, Austria, e-mail gottfried.brem@agrobiogen.de Acknowledgements: The equipment of Bioresources and Bioengineering Center of L.K. Ernst All-Russian Research Institute of Animal Husbandry was used. <...> Supported by the Russian Science Foundation, project ¹ 14-36-00039 A b s t r a c t Creating panels for parentage assignment based on the most informative SNPs (minor allele frequency, MAF≥ 0.3) is an important problem of the modern sheep breeding. <...> International Society of Animal Genetics (ISAG) recommends the parentage panel consisting of 88 autosomal SNPs, developed by the International Sheep Genomics Consortium. <...> However, selection of SNPs, which were included into the panel, was performed on the base of DNA profiles of North American, Australian and New Zealand sheep. <...> We have performed the whole genome SNP study in four local Russian sheep breeds — Romanov (ROM, = 22), Baikal’s fine-fleeced (ZBL, van short fat tailed (TUV, = 7), Buryat sheep Buubey (BUB, = 15), and Tu= 16) using Ovine SNP50k BeadChip. <...> Data were processed for the total number of markers (54241 SNPs) and for 88 autosomal SNPs, recommended by ISAG. <...> We estimated percentage of markers with MAF 0.3, mean MAF value, probability of identity (PI) and probabilities exclusion (P1, P2, P3) to evaluate the power of parentage panel for each of single breed and for entire sample. <...> The universality of the panel was evaluated by comparing the degree of genetic differentiation of breeds based on the study of the entire number of SNPs and panel ISAG. <...> After the quality control of the entire sample, we selected 47385 SNPs with mean MAF of 0.292±0.131 for the further analysis. <...> Analysis of the SNPs’ distribution depending on theirs MAF showed that most of the SNPs (81.8 %) were informative (MAF≥ 0.3). <...> Lower pairwise values of Fst based on 88 SNPs with the same character of genetic relations compared with those using whole genome SNP profiles shown high flexibility of ISAG panel. <...> PCA confirmed the low breed’s dependence of SNP panel by creating purely consolidated overlapping areas corresponding to different breeds. <...> Instead of some breeddependent character of DNA profiles of 88 autosomal SNPs, our <...>