Medvedeva and Vladimir S. Bondar Institute of Biophysics SB RAS 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia Received 07.05.2016, received in revised form 21.06.2016, accepted 14.08.2016 Pure mycelial culture of glowing mushroom is usually obtained from the fruiting body of the fungus or basidiospores. <...> Attempts to obtain pure cultures from glowing pieces of wood, leaf litter or soil are not always successful. <...> This paper describes the study of glowing wood samples which were collected on the Island Borneo in November and December 2013. <...> A method to isolate mycelium culture of glowing mushroom from the collected samples is proposed. <...> Control of the luminescence and selection of the samples is performed for the fi rst time with the system of video documentation, which can record the image of studied object and its luminescence. <...> The moisture maintenance of the studied sample is the most important condition for obtaining a pure culture of glowing mycelium. <...> The obtained pure culture of glowing mycelium is registered in the Culture Collection of Institute of Biophysics SB RAS with the number IBSO 2371. <...> When grown on solid media, the mycelium has long-lasting bright luminescence, the level and duration of which depends on the composition of the medium used. <...> Distinctive features of the studied culture are as follows: (a) the presence of local areas of the mycelium that change the intensity of the luminescence for short periods (several minutes) when it grown on solid media or natural wood; (b) the absence of bioluminescence in submerged culture. <...> After incubation of the culture <...>