In this, the biologicals based on Various groups of agents are involved in biological crop protection to control pests and genus possessing activity against harmful insects and phytopathogens is (Bt) dominate. <...> Serovar A Bt subspecies can form crystal endotoxins which are active against serovar B Bt subspecies attack the larvae of mosquitoes and black flies, and phytophagous and serovar C Bt subspecies are active against bacillus was identified. <...> A new serovar F (fungi) of this provide the assimilation of nutrient substrates and antibiosis against biocenosis partners. <...> Batsikol, the biological preparation based on (H10) with entomopathogenic action, was created at All-Russian Research Institute of Agricultural Microbiology (St. Petersburg). <...> Batsikol contains components of culture liquid, spores, insecticidal and fungicidal exo- and endotoxins, due to which it possesses multifunctional properties. <...> The article presents the mechanisms of entomopathogenic and antifungal action of microbial preparations based on Bt. <...> Results of testing Batsikol effectiveness against various pests and diseases in field trials and vegetation experiments are shown. <...> Liquid form of biological product was used in the study (spore titer of 3.5½109/ml). <...> Field and vegetation tests were carried out in 1994-2013 in different regions of Russia (Leningrad, Novosibirsk, Volgograd region, North Ossetia, Stavropol and Primorsky regions). <...> Different modes of application against phytopathogen were tested according to the type of parasitism and environmental characteristics of fungi (i.e., spraying, irrigation, seed treatment) In field experiments the efficacy of spraying strawberry plants against gray mold was 60-74 %. <...> Soil watering was used against Fusarium wilt on tomatoes and flax with efficacy of 74-87 % and 34-42 %, respectively. <...> When seeds were treated prior to sowing the efficacy was 66-71 % in case of soaking barley seeds against root rot, and 40-45 % while soaking potato tubers against damping-off. <...> Based on the tests conducted with Batsikol in different regions of Russia, the spectrum of its activities against wide range of phytophagous pests and pathogenic fungi was revealed on different crops. <...> The obtained data expand the understanding of Bt biology and, in particular, the action spectrum against various pests and diseases dangerous for many cultivated plants. <...> Presented materials allow considering preparations with a multifunctional activity. <...> Various <...>