The equipment of ARRIAM Center for Genome Technologies, Proteomics and Cell Biology (St. Petersburg) was used Among the 5 genera of tribe only species A b s t r a c t , Fed. is the least studied consisting of the (Stev.) Fed. <...> Vavilovia’s area of growth is limited by the highlands of Central and Eastern Caucasus, with only several known populations on the territories of Armenia, Dagestan, Norht Ossetia, Azerbaijan, Iran, Iraq, Siria, Turkey, and by the environmental conditions. <...> The only phylogenetic research of rhizobia isolated from the nodules of vavilovia from the Nortn Ossetian population demonstrated significance of both slow-growing and fast-growing microsymbionts’ specific and genetic diversity. <...> Three expeditions to the regions of Armenia, Dagestan and North Ossetia succeeded in finding and collecting plants of (Stev.) Fed. with its nodules, from which later rhizobia isolates were obtained. <...> We have chosen nineteen fast-growing isolates, derived from ten plants’ nodules, to identify their species affiliation, to trace geographical isolation and also to try to track down its genetic differences from rhizobia, which nodulate other plants of tribe transcribed spacer) fragment and . <...> To make this we sequenced ITS (internally gene, and made screening of the isolates for the presence of gene, which controls rhizobia host specificity. <...> Obtained sequences were used to calculate genetic distances between groups of rhizobia, i.e. different regions isolates (Armenia, Dagestan, North Ossetia) and isolates from different plant hosts (vavilovia, pea, clover). <...> Results of ITS sequencing showed that all strains involved in the analysis belong to (bv. dendrogram shows relatively high heterogeneity of isolates, but on ) species. <...> Difference in the structure of these dendrograms allows to assume that gene, chained with the genes of host specificity, can be easily transferred within the populations of , providing unlimited combinations of specificity to vavilovia with different variants of bacterial chromosome. <...> Comparison of genetic distances based on ITS-sequences for the isolates in this study shows tendency to geographic isolation between them. <...> Data on tances along with the presence of gene in the genomes of all study point out the presence of its host specificity within biovar tween strain origin and the genetic structure of among each group of , structure of ITS-loci reflect rhizobia adaptation to soil environment. <...> Keywords: Tribe family (син. reflects presence of highly specific <...>