AGRICULTURAL BIOLOGY, 2015, V. 50, ¹ 6, pp. 785-793 ISSN 2412-0324 (English ed. Online) (SEL’SKOKHOZYAISTVENNAYA BIOLOGIYA) ISSN 0131-6397 (Russian ed. Print) ISSN 2313-4836 (Russian ed. Online) Causative agents of especially dangerous viral infections — molecular genetics, immunogenisity, cultivation doi: 10.15389/agrobiology.2015.6.785rus doi: 10.15389/agrobiology.2015.6.785eng AFRICAN SWINE FEVER VIRUS GLYCOPROTEINS p54 AND CD2v IN THE CONTEXT OF IMMUNE RESPONSE MODULATION: BIOINFORMATIC ANALYSIS OF GENETIC VARIABILITY AND HETEROGENEITY K.A. MIMA, G.S. BURMAKINA, I.A. TITOV, A.S. MALOGOLOVKIN Federal Agency of Scientific Organizations, Pokrov, Petushinskii Region, Vladimir Province, 601120 Russia, e-mail mima89@ya.ru Acknowledgements: Supported by Russian Foundation for Basic Research, science project «Mol-a-ved» (15-34-20995). <...> A b s t r a c t which still remains the sole member of genus the most dangerous diseases of the animals from infecting soft ticks of the genus The African swine fever virus (ASFV) is a unique representative of Asfaviridae family, . <...> Genetic and phenotypic heterogeneity of ASF virus is one of the main reasons for the lack of vaccines against this dangerous transboundary disease. <...> In this work we present the analysis of structure and functions of the most variable glycoproteins ASFV p54 and CD2v using bioinformatics analysis and recombinant constructs expressed in mammalian cell cultures, the African green monkey cell culture COS-I and the human embryonal kidney cell culture HEK-293. <...> The index of variability of amino acid sequences for P54 and CD2v proteins was calculated by Simpson’s method. <...> The CD2v protein has variable region (N-terminal domain), which is highly glycosylated (28-30 sites) and located in the outer surface of the cell membrane. <...> The revealed differences in post-translational modifications and genetic variations of CD2v protein might explain the diversity of the hemadsorption phenomenon among ASF virus isolates. <...> In contrast, p54 protein has variable glycosylated extracellular and intracellular parts. <...> High level of differences in the nucleotide sequences of p54gene ( ) for various ASFV isolates may be the result of random mutations during virus evolution. <...> Characteristic antigenic properties of ASF virus isolates can obviously be due to found peculiar posttranslational <...>