Podbel’skogo, St. Petersburg, 196608 Russia, e-mail yuvkruglov@yandex.ru Acknowledgements: Supported by Russian Science Foundation (grant ¹ 14-26-00094) A b s t r a c t Study of the taxonomic and functional diversity of soil microorganisms association is of great theoretical significance for understanding the structure of the soil microbial community, the nature of the interaction of individual species of microorganisms belonging to this community, as well as their participation in the processes of soil formation and circulation of substances. <...> This article summarises a brief history of ideas about the functioning of soil microbial complex, providing transformation and mineralization of organic matter in soil formation processes. <...> This defines methodological difficulties and the significant variability of the results of the evaluation of soil microflora by various authors. <...> This review discusses the methodological approaches in determining the physiological diversity of soil microorganisms association. <...> Traditional methods of elective culture media in over a century allowed to reveal numerous physiological groups of microorganisms and developed an idea about their role in the cycle of matter, processes of soil formation and plant nutrition. <...> At the end of the 1990s a method of analysis of the carbon source utilization profiles (SUP) of natural microorganisms association by BIOLOG system used previously only in medical and general microbiology was proposed to study the physiological diversity for the test strain (J.L. Garland, A.L. Mills, 1991). <...> A number of modifications of this method (Eso-Plates, ECOLOGY and others) characterized by a set of organic substrates, which, as the authors suggest, is most likely present in natural environments were worked out. <...> Instrumentation of ECOLOG (M.V. Gorlenko, P.A. Kozhevin, 2005) allows to determine not only the range of organic substrates used by microorganisms, but also to quantify the consumption of each substrate. <...> For the processing and interpretation of a significant amount of information obtained in the course of the analysis of soil samples there are an apparatus of multidimensional mathematical statistics, cluster analysis, rank distribution, and ecological indexes of Shannon and Pielou. <...> SUP method (multisubstrat test) possesses a high performance, good resolution (104), a satisfactory reproducibility and is a high-tech and effective tool to assess the physiological diversity. <...> SUP reflects to some extent the potential of aerobic soil microorganisms using low molecular weight organic compounds in the catabolism. <...> Кeywords: association, physiological groups, physiological <...>