Draft whole-genome sequence of Bacillus altitudinis strain B-388, a producer of extracellular rnase. <...> BACterioPhAGes oF soil BACilli: neW MUltiVAlent PhAGe oF BACillUs AltitUDinis Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008, Kazan, Russian Federation Bacillus bacteria are soil saprophytes, facultative anaerobes that develop in the temperature range of 28-37°С. <...> Bacillus phages can be extensively used for phagotyping bacteria in the process of soil, water, and food monitoring. <...> Bacillus phages can also be used as vectors in horizontal gene transfer and potential therapeutic agents. thus, description of the biological diversity of the Bacillus phages can prove useful for further development of tools used in molecular biology and biomedicine. in this work, the scheme for isolation of soil bacteriophages was unified, which allowed 10 bacillus phages to be isolated from different types of soil. it was shown that the number of phages depended on the soil fertility, decreasing as the soil changes from black earth to brown earth to gray forest soil to uncontaminated urban soil to oil-contaminated urban soil. <...> A new polyvalent DnAcontaining bacteriophage srt01hs of B. altitudinis (it is also able to infect B. subtilis, B. cereus, B. pumilus, but not B. licheniformis and B. atrophaeus) was described in detail. it has a typical structure: total length, 360 nm; icosahedrons-shaped head, 100 nm in diameter. several phages simultaneously attack a B. altitudinis cell by increasing the level of intracellular low-molecular rnA. infection with the phage virtually eliminates the stationary growth phase of infected bacilli and <...>