TSYBANOV1 1All-Russian Institute of Veterinary Virology and Microbiology, Federal Agency of Scientific Organizations, 1, ul. <...> Vol’ginskii, Petushinskii Region, Vladimir Province, 601120 Russia, e-mail sindryakova.irina@yandex.ru; 2Kabardino-Balkarian Reference Center of Rosselkhoznadzor (Federal Service for Veterinary and Phytosanitary Surveillance), 1, ul. 9 Maya, Nalchik, Kabardino-Balkar Republic, 360051 Russia Received May 23, 2016 A b s t r a c t It is known that one of the ways to spread African swine fever (ASF) virus is alimentary way (e.g., through feeding animals with contaminated feed and slaughterhouse waste products not subjected to thermal treatment, not passed veterinary and sanitary examination, and derived from epidemiologically disadvantaged regions). <...> A variety of preserved food, such as corned beef, canned meat, bacon, etc., is made of pork products and by-products. <...> For the first time we carried out studies on infectious activity of ASF virus isolates circulating in the Russian Federation, in contaminated pig products, feed, as well as in samples intended for veterinary-sanitary supervision. <...> For inoculation we used ASFV isolate Volgograd-Kalach 2012 with the initial infectious titer of 7.5 lg HAU50/cm3 obtained from the National Collection of the SSINRRIVV&M of RAAS. <...> Three days after infection the animals were slaughtered, samples of internal organs and tissues were taken for virus isolation, and the pork was used for making corned beef, canned meat and salted lard. <...> Blood, containing ASF virus, was also used for contamination of a compound feed and drinking water. <...> To study effects of storage temperature on the virus infectious activity and genome detection, the samples were divided into aliquots and stored at 20-25 С (room temperature), 4-6 С (a common refrigerator); 16…20 С (freezer). <...> The temperatures selected for the storage of samples conform to the natural weather conditions at various seasons (20-25 С in the summer, 16…20 С in winter); 4-6 С is generally maintained in storerooms or stern room in the winter months. <...> Samples from the aliquots were taken with a 5 day intervals to determine the ASF virus titer and its genome fragments. <...> Virus isolation was carried out <...>