AGRICULTURAL BIOLOGY, 2016, V. 51, ¹ 3, pp. 392-400 ISSN 2412-0324 (English ed. Online) (SEL’SKOKHOZYAISTVENNAYA BIOLOGIYA) ISSN 0131-6397 (Russian ed. Print) ISSN 2313-4836 (Russian ed. Online) Elicitors and biological control in plant protection UDC 632.938.2:577.112.4:577.112.6 doi: 10.15389/agrobiology.2016.3.392rus doi: 10.15389/agrobiology.2016.3.392eng SEARCH FOR THE ACTIVE CENTER OF PEPTIDYL-PROLYL cys/trans ISOMERASE FROM Pseudomonas fluorescens RESPONSIBLE FOR THE INDUCTION OF TOBACCO (Nicotiana tabacum L.) PLANT RESISTANCE TO TOBACCO MOSAIC VIRUS V.G. DZHAVAKHIYA, T.M. VOINOVA, D.V. SHUMILINA All-Russian Research Institute of Phytopathology, Federal Agency of Scientific Organizations, 5, ul. <...> Received February 26, 2016 doi: 10.15389/agrobiology.2016.3.392eng A b s t r a c t The induction of pathogen resistance with biogenic elicitors is now considered as a promising plant protection method. <...> Elicitors obtained from fungal, bacterial, and oomycetic pathogens can be of peptide, glicoproteid, lipid, and oligosaccharide origin. <...> Based on this elicitor, a Messenger® preparation intended to protect plants from a wide range of pathogens was developed and commercialized in USA. <...> The MF3 protein able to induce the systemic resistance of plants to viral and fungal pathogens was isolated from the strain 197 of Pseudomonas fluorescens in the course of our earlier studies. <...> The full amino acid sequence of this protein was determined, and its high homology to the FKBP-type peptidyl-prolyl cys/trans isomerases of the same bacteria was demonstrated, so the isolated protein was called Pf197_PPIase. <...> We supposed that the active center of Pf197_PPIase responsible for its ability to induce the resistance is localized within the most conserved sequence of this protein. <...> The calculation of this sequence using a PROSITE bioinformational resource showed it contains sites composed of arginine and lysine and subjected to the tripsinolysis. <...> The preparations of Pf197_PPIase were obtained from the recombinant strain of Escherichia coli BL21(DE3)+plMF3 — the superproducer of Pf197_PPIase. <...> A Pf_29ac oligomer consisting of 29 amino acids and corresponding to the revealed conserved region was obtained by a chemical synthesis (Pushchino affiliated Branch of the Institute of Bioorganic Chemistry). <...> The further experiments showed that equimolar concentrations of Pf197_PPIase and Pf_29ac induced a similar level of resistance of tobacco plants to Tobacco Mosaic Virus (TMV). <...> These results <...>