AGRICULTURAL BIOLOGY, 2016, V. 51, ¹ 3, pp. 318-326 UDC 635.64:632.12:581.143:58.086 ISSN 2412-0324 (English ed. Online) (SEL’SKOKHOZYAISTVENNAYA BIOLOGIYA) ISSN 0131-6397 (Russian ed. Print) ISSN 2313-4836 (Russian ed. Online) doi: 10.15389/agrobiology.2016.3.318rus doi: 10.15389/agrobiology.2016.3.318eng COMPARATIVE ANATOMICAL AND MORPHOLOGICAL STUDIES OF THE EPIDERMAL AND CORTICAL PARENCHYMA HYPOCOTYL CELLS OF TWO TOMATO GENOTYPES (Solanum lycopersicum L.) UNDER SODIUM CHLORIDE STRESS in vitro L.R. BOGOUTDINOVA1, 2, G.B. BARANOVA1, E.N. BARANOVA1, M.R. KHALILUEV1, 2 1All-Russian Research Institute of Agricultural Biotechnology, Federal Agency of Scientific Organizations, 42, ul. <...> Timiryazevskaya, Moscow, 127550 Russia Acknowledgements: Supported by Russian Foundation for Basic Research, project ¹ 16-34-01331 (mol_a) Received January 9, 2016 A b s t r a c t Improving plant resistance to unfavorable environmental factors is one of the important tasks of modern agricultural production. <...> Understanding the physiological, biochemical, molecular and genetic adaptive mechanisms of tomato resistance to salinity as a complex trait is an essential part of most fundamental studies, the results of which are increasingly finding practical application during the last decade. <...> However, the data on the effect of sodium chloride on cell organization of different tomato tissues and organs are scarce. <...> Thus, the purpose of the study was to investigate cell organization of the epidermis and parenchyma cortical tissues of tomato hypocotyl (Solanum lycopersicum L. line YaLF and cultivar Rekordsmen) under chloride salinity in vitro. <...> Fragments of 10-12-day-old aseptically germinated tomato seedlings, whose roots have been removed, were transferred on root induction medium (1/2 МS, 2 % sucrose, 0.2 mg/l indole-3-butyric acid) supplemented with 0-250 mМ NaCl. <...> After 8 days in culture, middle part of hypocotyls were excised from rooted seedlings and prepared for light microscopy. <...> Histological examination revealed significant differences between genotypes in shape and average cross-sectional areas of the epidermal and cortical parenchyma cells of hypocotyl. <...> The addition of Na+ and Cl ions to culture medium significantly affected the size of the intercellular spaces in the cortical parenchyma as well as the average cross-sectional areas and shape epidermal and cortical parenchyma hypocotyl cells of both tomato <...>