Biology 2 (2015 8) 236-253 ~ ~ ~ УДК 577.12 Fabrication and Degradation of Electrospun Polyhydroxyalkanoate Film Lee Joyyia Nanthini Sridewib Ken-ichi Kasuyac a , Amirul Al-Ashraf Abdullaha and Kumar Sudesha , , * School of Biological Sciences University Sains Malaysia Penang, 11800, Malaysia bNational Defence University of Malaysia Kuala Lumpur, 57000, Malaysia c 1-5-1 Tenjin, Kiryu, Gunma 376-8515, Japan Received 19.01.2015, received in revised form 20.02.2015, accepted 16.05.2015 Polyhydroxyalkanoates (PHAs) are a family of biopolymers with good biodegradability. <...> Poly(3hydroxybutyrate) [P(3HB)], poly(3-hydroxybutyrate-co-25 mol % 4-hydroxybutyrate) [P(3HBco-25 mol % 4HB)] and poly(3-hydroxybutyrate-co-75 mol % 4-hydroxybutyrate) [P(3HB-co-75 mol % 4HB)] were fabricated using the electrospinning technique to obtain fi bers. <...> Electrospun P(3HB) showed formation of fi bers when 30 kV voltage was applied to 4 % P(3HB) extruded at 60 μL/min with prior heating for 15 min at 60 °C. <...> Fabricated P(3HB-co-4HB) showed a continuous polymer mat with embedded beads formation. <...> P(3HB) fabricated at different time of electrospinning (5, 10, 15 and 20 min) and concentrations (1 %, 2 %, 3 % and 4 %) subjected to in vitro enzymatic degradation by PHA depolymerase showed decrease in polymer weight. <...> The highest rate of degradation was exhibited by 2 % P(3HB) electrospun for 15 min. Films of 1 % P(3HB-co-25 mol % 4HB) and 1 % P(3HBco-75 mol % 4HB) subjected to in vitro lipase degradation also exhibited decrease in polymer weight. <...> P(3HB-co-75 mol % 4HB) fi lms showed signifi cant decrease in weight compared to P(3HB-co-25 mol % 4HB). <...> Degraded P(3HB) had fi bril-like structures whereas P(3HB-co-4HB) surface structure became more porous. <...> Environmental degradation of these polymers was successful with P(3HB-co4HB) being better degraded. <...> All rights reserved * Corresponding author E-mail address: ksudesh@usm.my # 236 # Division of Molecular Science Gunma University Lee Joyyi, Nanthini Sridewi… Fabrication and Degradation of Electrospun Polyhydroxyalkanoate Film Изготовление и деградация пленок <...>