SEL’SKOKHOZYAISTVENNAYA BIOLOGIYA [AGRICULTURAL BIOLOGY], 2015, V. 50, ¹ 1, pp. 46-54 ISSN 2313-4836 (Online) doi: 10.15389/agrobiology.2015.1.46rus doi: 10.15389/agrobiology.2015.1.46eng MICROSATELLITE LOCI POLYMORPHISM IN RUSSIAN BLACK CURRANT ( L.) VARIETIES FROM COLLECTION OF ALL-RUSSIAN RESEARCH INSTITUTE OF BREEDING FRUIT CROPS A.V. PIKUNOVA1, S.D. KNYAZEV1, A.Yu. <...> Gubkina, Moscow, 119991 Russia, e-mail iogen@vigg.ru Supported by Russian Science Foundation (project ¹ 14-1600127) and Ministry of Education and Science of the Russian Federation (agreement ¹ 8820) A b s t r a c t Black currant is the main berry crop in Russia. <...> Application of DNA markers as a modern approach in dealing with germplazm is intensively used abroad in works on black currant plants. <...> We are the first in Russia who used SSR DNA markers in studying black currant gene pool. <...> In this paper we report genotyping 27 black currant accessions from the collection of the All-Russian Research Institute of Breeding Fruit Crops, including 16 varieties originated from this institute, on 14 microsatellite loci. <...> Electrophoresis in 6 % denaturing PAAG followed by staining with silver nitrate was used for separation of PCR products. <...> All tested SSR loci, except MS06g03, have been found to be polymorphic. <...> On average 4.9 alleles were amplified per locus. <...> Three fragments have been amplified on DNA of some accessions at one SSR locus that is probably due to the duplication of these microsatellite loci in genome of these accessions. <...> A total of 66 % of all amplified fragments were rare alleles with a frequency of occurrence equal to or less than 0.2. <...> Combinations of alleles at one locus allowed to distinguish from 3 (g2-H21, e1-O21) to 15 (g2-G12) genotypes. <...> The minimum set of four loci (e4-D03, g1-M07, g1-E03, g2-B20) allowed to distinguish all the tested accessions, i.e. unique multilocus profile has been found for each sample. <...> Pairwise genetic similarity coefficients ranged from 0.11 (between Odzhebin and Sharovidnaya varieties) to 0.95 (between Govtva and Rtishchevskaya varieties) and averaged 0.346. <...> The dendrogram shows a few clusters with high bootstrap support. <...> Thus, the varieties Ocharovanie (¹ 1168 ½ Ekzotika variety) and Ekzotika had no common alleles in three loci (e4D03, g1-E03, g2-B20). <...> The varieties Odzhebin and Binar (Odzhebin ½ Naryadnaya) had <...>