Timiryazeva, Zhodino, 222160 Republic of Belarus, e-mail tinck@inbox.ru Supported by the Ministry of Education and Science of the Russian Federation A b s t r a c t Anthracnose is one of the fungal diseases of the narrow-leafed lupine ( L.) caused by . <...> Resistance to anthracnose is not absolute in character, as the plants with high resistance can be affected by the pathogen but in less extent than those non-resistant. <...> Current approach in breeding anthracnose-tolerant lupine is based on combination of non-allele genes of resistance in a single genotype. <...> Specific DNA markers are being developed which are linked to the genes of resistance and can be used for rapid and effective selection of resistant plants, but prior to their application the efficacy of DNA marker should be specifically tested with the breeding material of interest to avoid false positive responces. <...> The AnSeq3 and AnSeq4 DAN markers flanking gene at 0.9 cM distance are considered the closest to it (H. Yang et al., 2012). <...> In this article we report the possibility of using DNA markers AnSeq3 and AnSeq4, the single nucleotide polymorphisms (SNPs), in selecting forms resistant to anthracnose among varieties of narrow-leafed lupine. <...> A total of 50 Russian, Belarusian, Polish and Australian varieties and samples were tested to detect the allele DNA markers of susceptibility or resistance to anthracnose. <...> The list of tested plants, PCR mix composition and protocol are specified. <...> Polyacrylamide gel electrophoresis was used for visualization of the allele markers. <...> The Australian varieties resistant and susceptible to anthracnose were used as a control for AnSeq3 and AnSeq4 alleles. <...> DNA fragments of 92 and 87 base pairs corresponding to the markers AnSeq3 and AnSeq4, respectively, were obtained for all 50 breeding samples included in the study. <...> For 13 Russian and 10 Polish varieties the marker alleles of susceptibility to anthracnose were detected. <...> For BGB-6 Belarusian sample the resistance alleles were identified by AnSeq3 and AnSeq4 markers, and for Myrtan variety only the AnSeq3-specific pattern was shown. <...> Anthracnose is an infectious disease of many agricultural crops, includL. <...> At the same time, it should be noted that this resistance is not absolute, but relative, because plant specimens with high resistance can be affected by the pathogen, but in less extent than those considered <...>