Agricultural Biology / №1 2016

Bacillus thuringiensis STRAINS FROM NATURAL SOURCES IN THE LENINGRAD REGION: ISOLATION AND IDENTIFICATION (150,00 руб.)

Первый автор	ERMOLOVA
Страниц	7

150,00р

ID	365478
Аннотация	Recently crystal-forming bacilli of thuringiensis group are considered the main microbial producers of insecticides. For these bacilli the high adaptability is characteristic leading to wide distribution of these anaerobic spore-forming bacteria in nature. The same Bacillus thuringiensis subspecies and variants were isolated on different continents regardless the presence and prevalence or absence of the host insects of this entomopathogen. In different countries and regions the researchers are searching for new B. thuringiensis isolates. In the paper the data are represented on B. thuringiensis isolation from natural substrates in the territory of Leningrad province. A total of 24 samples of soil, litter, water, silt, sick and died insects have been collected. The samples were cultivated on fish agar. Among more than 3,000 colonies, 62 ones with specific morphology were found. By microscopy with black aniline dye a total of 12 isolates of 62 isolates tested were found out to form both spores and differently shaped crystals of the endotoxin. The microorganisms were selected with regard to entomocidal and larvicidal activity and identified using H. De Barjac, A.A. Bonnefoi (1968) and O. Lysenko (1985) schemes. The investigation made it possible to classify isolates as B. thuringiensis of Н1 (var. thuringiensis, isolates ¹¹ 12, 20, 40, 41), Н3а3в (var. kurstaki, isolates ¹¹ 15, 29, 49) and Н14 (var. israelensis, isolates ¹¹ 14, 25, 33, 38, 44) serovars. With regard to biological properties (production of acetyl methyl carbonate, lecithinase, pigment, -exotoxin; pellicle in broth culture; sucrose, mannose, cellobiose, salicin fermentation; starch degradation; proteolytic activity) these isolates are close to standard strains. Isolates are characterized by high productivity, entomocidal and larvicidal activity and can be used as producers of biologicals against insects and larvae. In the isolates of BtH1, BtH3a3b and BtH14 serovars the titers varied as 2.42½109-2.78½109; 1.85½109-2.15½109 and 2.65½109-3.28½109 CFU/ml, respectively. The activity against Leptinotarsa decemlineata Say larvae in isolates ¹¹ 12, 41 of the BtH1 serovar was the same as in standard strain BtH1 with LD50 at 0.19 %. Entomocidal activity of the isolates ¹¹ 15, 29 and 49 of the BtH3a3b serovar expressed as LD50 for Ephestia kuehniella of the 2nd instar was 0.88; 0.82 and 0.92 %, respectively, while in the standard strain BtH3a3b the LD50 was 0.86 %. In the isolates ¹¹ 33, 44 of the BtH14 serovar the titer was the same as in the standard strain, and the activity was even higher compared to the standard. In the isolates ¹¹ 33, 44 the LD50 for the 4th instar Aedes aegypti larvae was 0.17½10-3 and 0.16½10-3 %, respectively, when in standard strain BtH14 it was 0.18½10-3 %. Thus, a total of 12 of the isolates which have been identified as B. thuringiensis are close to the type isolates on their biological characteristics and promising as producers of biologics with insecticidal action.
УДК	631.46:579.64:632.937.12(470.23)

ERMOLOVA, V.P. Bacillus thuringiensis STRAINS FROM NATURAL SOURCES IN THE LENINGRAD REGION: ISOLATION AND IDENTIFICATION / V.P. ERMOLOVA // Agricultural Biology .— 2016 .— №1 .— С. 113-119 .— URL: https://rucont.ru/efd/365478 (дата обращения: 19.04.2024)

Предпросмотр (выдержки из произведения)

AGRICULTURAL BIOLOGY, 2016, V. 51, ¹ 1, pp. 128-136 ISSN 2412-0324 (English ed. Online) (SEL’SKOKHOZYAISTVENNAYA BIOLOGIYA) ISSN 0131-6397 (Russian ed. Print) ISSN 2313-4836 (Russian ed. Online) Ecological approach to developing microbial biologicals doi: 10.15389/agrobiology.2016.1.128rus doi: 10.15389/agrobiology.2016.1.128eng STRAINS FROM NATURAL SOURCES IN THE LENINGRAD REGION: ISOLATION AND IDENTIFICATION V.P. ERMOLOVA Federal Agency of Scientific Organizations, 3, sh. <...> Podbel’skogo, St. Petersburg, 196608 Russia, e-mail Ermolovavalya1940@mail.ru Acknowledgements: Supported by the Ministry of Education and Sciences of the Russian Federation (Agreement ¹ 14.604.21.0024, RFMEFI60414Х0024) A b s t r a c t Recently crystal-forming bacilli of group are considered the main microbial producers of insecticides. <...> For these bacilli the high adaptability is characteristic leading to wide distribution of these anaerobic spore-forming bacteria in nature. <...> The same subspecies and variants were isolated on different continents regardless the presence and prevalence or absence of the host insects of this entomopathogen. <...> In the paper the data are represented isolation from natural substrates in the territory of Leningrad province. <...> A total of 24 samples of soil, litter, water, silt, sick and died insects have been collected. <...> Among more than 3,000 colonies, 62 ones with specific morphology were found. <...> By microscopy with black aniline dye a total of 12 isolates of 62 isolates tested were found out to form both spores and differently shaped crystals of the endotoxin. <...> The microorganisms were selected with regard to entomocidal and larvicidal activity and identified using H. De Barjac, A.A. Bonnefoi (1968) and O. Lysenko (1985) schemes. <...> With regard to biological properties (production of acetyl methyl carbonate, lecithinase, pigment, -exotoxin; pellicle in broth culture; sucrose, mannose, cellobiose, salicin fermentation; starch degradation; proteolytic activity) these isolates are close to standard strains. <...> Isolates are characterized by high productivity, entomocidal and larvicidal activity and can be used as producers of biologicals against insects and larvae. <...> The activity against Say larvae in isolates ¹¹ 12, 41 of the BtH1 serovar was the same as in standard strain BtH1 with LD50 at 0.19 %. <...> In the isolates ¹¹ 33, 44 of the BtH14 serovar the titer was the same as in the standard strain, and the activity was <...>

Облако ключевых слов *

analysis dispersion bth1 bth14 bth3a3b entomocidal exotoxin insect interval confidence isolate larva larva mosquito larvicidal ld50 rate death salicin serovar serovars specific morphology standard strain strain bacillus sulphide hydrogen вtн1 вtн14

* - вычисляется автоматически


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